Interplay among photoreceptors determines the strategy of coping with excess light in tomato.

This study investigates photoreceptor's role in the adaption of photosynthetic apparatus to high light (HL) intensity by examining the response of tomato wild type (WT) (Solanum lycopersicum L. cv. Moneymaker) and tomato mutants (phyA, phyB1, phyB2, cry1) plants to HL. Our results showed a photoreceptor-dependent effect of HL on the maximum quantum yield of photosystem II (Fv /Fm ) with phyB1 exhibiting a decrease, while phyB2 exhibiting an increase in Fv /Fm . HL resulted in an increase in the efficient quantum yield of photosystem II (ΦPSII ) and a decrease in the non-photochemical quantum yields (ΦNPQ and ΦN0 ) solely in phyA. Under HL, phyA showed a significant decrease in the energy-dependent quenching component of NPQ (qE ), while phyB2 mutants showed an increase in the state transition (qT ) component. Furthermore, ΔΔFv /Fm revealed that PHYB1 compensates for the deficit of PHYA in phyA mutants. PHYA signaling likely emerges as the dominant effector of PHYB1 and PHYB2 signaling within the HL-induced signaling network. In addition, PHYB1 compensates for the role of CRY1 in regulating Fv /Fm in cry1 mutants. Overall, the results of this research provide valuable insights into the unique role of each photoreceptor and their interplay in balancing photon energy and photoprotection under HL condition.

Genotype-dependent Strategies to "Overcome" Excessive Light: Insights into Non-Photochemical Quenching under High Light Intensity.

High light (HL) intensities have a significant impact on energy flux and distribution within photosynthetic apparatus. To understand the effect of high light intensity (HL) on the HL tolerance mechanisms in tomatoes, we examined the response of the photosynthesis apparatus of 12 tomato genotypes to HL. A reduced electron transfer per reaction center (ET0 /RC), an increased energy dissipation (DI0 /RC) and non-photochemical quenching (NPQ), along with a reduced maximum quantum yield of photosystem II (FV /FM ), and performance index per absorbed photon (PIABS ) were common HL-induced responses among genotypes; however, the magnitude of those responses was highly genotype-dependent. Tolerant and sensitive genotypes were distinguished based on chlorophyll fluorescence and energy-quenching responses to HL. Tolerant genotypes alleviated excess light through energy-dependent quenching (qE ), resulting in smaller photoinhibitory quenching (qI ) compared to sensitive genotypes. Quantum yield components also shifted under HL, favoring the quantum yield of NPQ (ՓNPQ ) and the quantum yield of basal energy loss (ՓN0 ), while reducing the efficient quantum yield of PSII (ՓPSII ). The impact of HL on tolerant genotypes was less pronounced. While the energy partitioning ratio did not differ significantly between sensitive and tolerant genotypes, the ratio of NPQ components, especially qI , affected plant resilience against HL. These findings provide insights into different patterns of HL-induced NPQ components in tolerant and sensitive genotypes, aiding the development of resilient crops for heterogeneous light conditions.

Artificial neural network (ANN)-based algorithms for high light stress phenotyping of tomato genotypes using chlorophyll fluorescence features.

High light (HL) is a common environmental stress directly imposes photoinhibition on the photosynthesis apparatus. Breeding plants for tolerance against HL is therefore highly demanded. Chlorophyll fluorescence (ChlF) is a sensitive indicator of stress in plants and can be evaluated using OJIP transients. In this study, we compared the ChlF features of plants exposed to HL (1200 µmol m-2 s-1) with that of control plants (300 µmol m-2 s-1). To extract the most reliable ChlF features for discrimination between HL-stressed and non-stressed plants, we applied three artificial neural network (ANN)-based algorithms, namely, Boruta, Support Vector Machine (SVM), and Recursive Feature Elimination (RFE). Feature selection algorithms identified multiple features but only two features, namely the maximal quantum yield of PSII photochemistry (FV/FM) and quantum yield of energy dissipation (ɸD0), remained consistent across all genotypes in control conditions, while exhibited variation in HL. Therefore, considered reliable features for HL stress screening. The selected features were then used for screening 14 tomato genotypes for HL. Genotypes were categorized into three groups, tolerant, semi-tolerant, and sensitive genotypes. Foliar hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents were measured as independent proxies for benchmarking selected features. Tolerant genotypes were attributed with the lowest change in H2O2 and MDA contents, while the sensitive genotypes displayed the highest magnitude of increase in H2O2 and MDA by HL treatment compared to the control. Finally, a FV/FM higher than 0.77 and ɸD0 lower than 0.24 indicates a healthy electron transfer chain (ETC) when tomato plants are exposed to HL.

Synergistic Effects of Melatonin and Gamma-Aminobutyric Acid on Protection of Photosynthesis System in Response to Multiple Abiotic Stressors.

GABA (gamma-aminobutyric acid) and melatonin are endogenous compounds that enhance plant responses to abiotic stresses. The response of Vicia faba to different stressors (salinity (NaCl), poly ethylene glycol (PEG), and sulfur dioxide (SO2)) was studied after priming with sole application of GABA and melatonin or their co-application (GABA + melatonin). Both melatonin and GABA and their co-application increased leaf area, number of flowers, shoot dry and fresh weight, and total biomass. Plants treated with GABA, melatonin, and GABA + melatonin developed larger stomata with wider aperture compared to the stomata of control plants. The functionality of the photosynthetic system was improved in primed plants. To investigate the photosynthetic functionality in details, the leaf samples of primed plants were exposed to different stressors, including SO2, PEG, and NaCl. The maximum quantum yield of photosystem II (PS II) was higher in the leaf samples of primed plants, while the non-photochemical quenching (NPQ) of primed plants was decreased when leaf samples were exposed to the stressors. Correlation analysis showed the association of initial PIabs with post-stress FV/FM and NPQ. Stressors attenuated the association of initial PIabs with both FV/FM and NPQ, while priming plants with GABA, melatonin, or GABA + melatonin minimized the effect of stressors by attenuating these correlations. In conclusion, priming plants with both GABA and melatonin improved growth and photosynthetic performance of Vicia faba and mitigated the effects of abiotic stressors on the photosynthetic performance.

Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers.

A new protocol for in vitro regeneration through direct somatic embryogenesis for two muskmelon cultivars (Cucumis melo L., "Mashhadi" and "Eivanaki") is reported. Somatic embryos were obtained culturing 4- and 8-day-old cotyledons, seeds, and hypocotyls on Murashige and Skoog medium supplemented with three different hormonal combinations never tested so far for melon (naphthoxyacetic acid (NOA) + thidiazuron (TDZ), NOA + 6-banzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D) + N-(2-chloro-4-pyridyl)-N'-phenylurea (4-CPPU)). Results were compared with those obtained when explants were cultivated in the presence of 2,4-D + BAP, previously used on melon. Embryogenesis occurred more successfully in 4-day-old cotyledons and seeds than hypocotyls and 8-day-old cotyledons. The best result was achieved with NOA + BAP. Genotypes significantly affected embryogenesis. The number of embryos in "Eivanaki" was significantly higher than that in "Mashhadi." Embryo proliferation when explants were maintained in jars (9.3%) was found to be higher compared to that in petri dishes. For the first time, genetic stability of regenerated melon plants was evaluated using inter-simple sequence repeat markers. Polymerase chain reaction (PCR) products demonstrated a total of 102 well-resolved bands, and regenerants were 93% similar compared to the mother plant. Somaclonal changes during embryogenesis were evaluated by flow cytometry, showing 91% of the same patterns in regenerated plants. The results suggest that the new hormone components are effective when applied for in vitro embryogenesis of muskmelon as they show a high frequency in regeneration and genetic homogeneity.